Fig. 2

CB1 promoted the OCR, MMP and expressions of Nrf1 and Nrf2 in hBMSCs. A–G Knockdown of CB1 by CB1 shRNA1 inhibited the OCR in BMSCs. The Seahorse XFe24 analyzer showed the results of OCR curves (A), the basal OCR (B), ATP production (C), maximal respiration (D), spare respiratory capacity (E), proton leak (F) and non-mitochondrial oxygen consumption (G). H–N Role of over-expressing CB1 on the OCR in BMSCs. The Seahorse XFe24 analyzer showed the results of OCR curves (H), the basal OCR (I), ATP production (J), maximal respiration (K), spare respiratory capacity (L), proton leak (M) and non-mitochondrial oxygen consumption (N). O The JC-10 results in CB1 over-expressing BMSCs. CCCP was used as a positive control. Scale bar: 50 μm. P–S The Real-time RT-PCR results of Nrf1 (P, R), and Nrf2 (Q, S) in BMSCs. GAPDH was used as an internal control. Student’s t test was performed to determine statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01