Fig. 4

Over-expression of CB1 promoted the OCR in hBMSCs under TNF-α and INF-γ stimulation. A–G 10 ng/ml TNF-α was used to treat BMSCs for 24 h. The Seahorse XFe24 analyzer showed the results of OCR curves (A), the basal OCR (B), ATP production (C), maximal respiration (D), spare respiratory capacity (E), proton leak (F) and non-mitochondrial oxygen consumption (G). H–N 100 ng/ml INF-γ was used to treat BMSCs for 24 h. The Seahorse XFe24 analyzer showed the results of OCR curves (H), the basal OCR (I), ATP production (J), maximal respiration (K), spare respiratory capacity (L), proton leak (M) and non-mitochondrial oxygen consumption (N). The One-way ANOVA was performed to determine statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01