Fig. 5

Over-expression of CB1 promoted the MMP and expressions of Nrf1 and Nrf2 in hBMSCs under TNF-α and INF-γ stimulation. A 10 ng/ml TNF-α was used to treat BMSCs for 24 h, the JC-10 results of BMSCs. CCCP was used as a positive control. Scale bar: 50 μm. B, C 10 ng/ml TNF-α was used to treat BMSCs for 4 h, the Real-time RT-PCR results of Nrf1 (B) and Nrf2 (C) expressions in BMSCs. D 100 ng/ml INF-γ was used to treat BMSCs for 24 h, the JC-10 results of BMSCs. CCCP was used as a positive control. Scale bar: 50 μm. E, F 10 ng/ml TNF-α was used to treat BMSCs for 4 h, the Real-time RT-PCR results of Nrf1 (E) and Nrf2 (F) expressions in BMSCs. GAPDH was used as an internal control. The One-way ANOVA was performed to determine statistical significance. Error bars represent SD (n = 3). *P ≤ 0.05; **P ≤ 0.01