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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Sin3a drives mesenchymal-to-epithelial transition through cooperating with Tet1 in somatic cell reprogramming

Fig. 6

Sin3a cooperates with Tet1 to promote MET and somatic cell reprogramming. A The cell morphology of wild-type Sin3a or Sin3a mutants (Sin3aF147A and Sin3aF182A) overexpression in shSin3a-1 cells at Day 5 and Day 8 of MEF reprogramming. Black arrows indicate the representative cell–cell contact morphology. B qRT-PCR analysis of the expression changes of epithelial genes at Day 8 in shSin3a-1 cells overexpressing wild-type Sin3a or Sin3a mutants. C Western blotting analysis of SIN3A, E-cadherin, and β-catenin expression at Day 8 of MEF reprogramming. GAPDH was used as loading control. D Flow cytometry detection of the percentage of E-cadherin and β-catenin positive cells at Day 8 of reprogramming. E qRT-PCR analysis for the expression changes of mesenchymal genes at Day 8 of reprogramming. F qRT-PCR analysis of pluripotency marker expression at Day 12 of MEF reprogramming. G–H Reprogramming efficiency was measured by AP staining. Representative images (G) and the number of AP+ colonies (H) are shown. Significance was estimated by student’s unpaired t test. Error bars represent SD (n = 3 independent experiments). *P < 0.05, **P < 0.01, and ***P < 0.001 versus pMKO.1-treated group with fugw vector (the white column), #P < 0.05, ##P < 0.01, and ###P < 0.001 versus shSin3a-1-treated group with fugw vector (the blue column), $P < 0.05, $$P < 0.01, and $$$P < 0.001 versus the wild-type Sin3a overexpression group (the red column)

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