Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: First-in-human autologous oral mucosal epithelial sheet transplantation to prevent anastomotic re-stenosis in congenital esophageal atresia

Fig. 2

Fabrication and quality control tests for cultured autologous oral mucosal epithelial cell sheets. A Collection of oral mucosal tissue from the patient. B Buccal mucosal tissue harvested from the oral cavity of this patient. The superficial area of the mucosal tissue was approximately 0.8 cm2. C Cellular morphology of oral mucosal cells before seeding onto temperature-responsive culture inserts. Cells marked with white arrows were terminally differentiated epithelial cells which were not counted as viable cells for determining seeding density of the cells. Scale bar is 200 µm. D Cellular morphology of the oral mucosal cells 8 days after seeding. Scale bar is 200 µm. E Cultured autologous oral mucosal epithelial cell sheet for quality control testing for quantification of cellular density, viability, and percentage of epithelial cells. F Histogram of flow cytometry results for determining percentage of epithelial cells in the cell sheet by detection of cytokeratin-positive cells. Percentage of cytokeratin-positive cells determined to be epithelial cells was 99.1% in the epithelial cell sheet. G Cellular morphology of the oral mucosal cells before transportation to the hospital. Scale bar is 200 µm

Back to article page