Fig. 2

Chronological macroscopic (A) and microscopic changes (B) of the rat liver during the SLES-based decellularization process. The change of liver color from red to transparent during the decellularization procedure can be observed. DNA quantification (C) showed a significant reduction in the DNA content after decellularization (data are expressed as the mean ± standard error of the mean (SEM), N = 3 per group, *Indicates significant difference, (P = 0.0002). Hoechst (D), Hematoxylin and eosin (E) staining of decellularized liver confirms nuclei depletion. Scanning electron microscopy of the cut of edge (F) and surface (G) of decellularized scaffolds shows that the 3D ultrastructure remains intact