Fig. 4
From: Cell maturation influences the ability of hESC-RPE to tolerate cellular stress
Phagocytosis assay of hESC-RPE at time points of 3 weeks and 12 weeks. a Each image consists of a laser scanning confocal microscopy z-maximum intensity projection (z-MIP) and xz cross-sections (MIP from 20 sections) with POS particles (opsin staining) shown in green and F-actin (phalloidin staining) shown in red. Scale bar is 20 µm. POS particles inside the cells were calculated from xz cross-sections and are shown as b number of internalized POS particles per field (total field number 10–15 from 2 to 3 experiments) and c number of internalized POS particles per cell. Each field contains ca. 100 cells. Internalized POS particles were calculated as in [12]. Data represent means ± SD. **p < 0.01