Fig. 5
From: Cell maturation influences the ability of hESC-RPE to tolerate cellular stress
Ca2+ signaling of control, MG132 and H2O2 treated hESC-RPE cells at time points of 3 and 12 weeks. Images represent a relative number of cells that respond to ATP stimulus at both timepoints, and relative maximum amplitudes at timepoints of b 3 weeks and c 12 weeks. Representative response curves at the timepoints of d 3 weeks and e 12 weeks. All results are conducted from 2 to 5 measurements. All data represents means ± SD. *p < 0.05 and ***p < 0.001. f Representative laser scanning confocal microscopy z-maximum intensity projections (z-MIP) and yz cross sections (MIP from 10 sections) of expression and localization of P2Y2 receptor. Scale bar is 20 µm