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Table 1 Culture conditions and gradient load

From: Identification of functional pathways for regenerative bioactivity of selected renal cells

Cell prep

Seeding density

Culture time

Final confluency (%)

Gradient load

RK086

17.5 e6/flask

3d 21% O2

1d 2% O2

100

72.8 e6

RK087

15 e6/flask

2d 21% O2

1d 2% O2

85

91 e6

RK097

19.3 e6/flask

2d 21% O2

1d 2% O2

85

92.5 e6

  1. Gradient load refers to the number of PreG cells loaded onto the iodixanol gradient for separation into SRC component sub-populations B1–B5