Fig. 2

NDRG1 suppressed osteogenic differentiation of primary BMSCs. Overexpression (A) or silencing (E) of Ndrg1 in primary BMSCs after infection of Ndrg1 expression lentivirus or shRNA expression lentivirus was verified using qRT-PCR. Primary BMSCs overexpressing or underexpressing Ndrg1 were induced to allow osteogenic differentiation. The effects of Ndrg1 overexpression (B–D) or silencing (F–H) on osteogenic differentiation were examined. ALP staining and alizarin red staining were performed in differentiated osteoblasts 14 days and 21 days, respectively, after osteogenic treatment (B, F). The mRNA (C, G) and protein (D, H) levels of osteogenic factors were measured 72 h after osteogenic treatment. Values represent mean ± SD, n = 3. *Significant versus control LV, p < 0.05