Fig. 3

NDRG1 stimulated adipogenic differentiation of primary BMSCs. Primary BMSCs infected with Ndrg1 overexpression or shRNA lentivirus were induced to allow adipogenic differentiation. The effects of Ndrg1 overexpression (A–D) or silencing (E–H) on adipogenic differentiation were examined. Oil-red O staining was performed in differentiated adipocytes 5 days after adipogenic treatment (A, E) and the stain extracted with isopropanol was measured at 520 nm by spectrophotometry (B, F). The mRNA (C, G) and protein (D, H) levels of adipogenic factors were measured 48 h and 72 h, respectively, after adipogenic treatment. Image scale in A, E: 100 μm. Values represent mean ± SD, n = 3. *Significant versus control LV, p < 0.05