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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: N-myc downstream regulated gene 1 suppresses osteoblast differentiation through inactivating Wnt/β-catenin signaling

Fig. 4

NDRG1 interacted with LRP6 and inactivated canonical Wnt signaling. The nuclear translocation of β-catenin was examined in ST2 cells transfected with Ndrg1 expression construct (A) or siRNA (B). The protein levels of the major components of canonical Wnt signaling were measured using Western blotting in undifferentiated ST2 72 h after overexpression of Ndrg1 (C), or silencing of Ndrg1 (D). Co-IP was done to detect the interaction between NDRG1 and LRP6 (E). ST2 cells transfected with Ndrg1 expression construct or the vector were treated with cycloheximide and the amount of LRP6 was detected by Western blotting (F). Image scale in A, B: 50 μm. Values represent mean ± SD, n = 3. *Significant versus vector (C), or control siRNA (D), or 0 h (F), p < 0.05

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