Fig. 6

Silencing of Ndrg1 prevented the inactivation of canonical Wnt signaling and ameliorated the impaired differentiation of BMSCs from ovariectomized mice. qRT-PCR was done to verify the downregulation of Ndrg1 mRNA in BMSCs depleted of Ndrg1 (A). BMSCs were isolated and cultured, and proteins were extracted and subjected to Western blotting (B). BMSCs were induced to allow osteogenic (C–E) or adipogenic differentiation (F–I). ALP staining and alizarin red staining were performed in differentiated osteoblasts 14 days and 21 days, respectively, after osteogenic treatment (C). The mRNA (D) and protein (E) levels of osteogenic factors were measured 72 h after osteogenic treatment. Oil-red O staining was performed in differentiated adipocytes 5 days after adipogenic treatment (F) and the stain extracted with isopropanol was measured at 520 nm by spectrophotometry (G). The mRNA (H) and protein (I) levels of adipogenic factors were measured 48 h and 72 h, respectively, after adipogenic treatment. Image scale in F: 100 μm. Data are mean ± SD, n = 3. *p < 0.05 versus Sham/Ctrl siRNA. ^#p < 0.05 versus OVX/Ctrl siRNA