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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: G protein-coupled receptor kinase 3 modulates mesenchymal stem cell proliferation and differentiation through sphingosine-1-phosphate receptor regulation

Fig. 2

Osteogenic expression markers increase in MSC cultures during osteogenic differentiation relative to housekeeping gene IDUA. qRT-PCR of osteogenic differentiation markers alkaline phosphatase, osterix, osteonectin, and osteocalcin from BmMSC ex vivo cultures in osteogenic differentiation media over time (days 0–17). Left panels are comparing Grk3-/- to WT BmMSC cultures (n = 6, passages 10–15), and right panels are comparing Grk3-shRNA knockdown to nontarget shRNA control BmMSC cultures (n = 4, passages 11,12). * P ≤ 0.05 ** P ≤ 0.01

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