Table 1 Summary of recent findings on dissecting the heterogeneous stromal compartment of bone marrow using scRNA-seq
From: Single-cell RNA sequencing to track novel perspectives in HSC heterogeneity
Publication | Sequencing objects | Key findings | Dataset |
|---|---|---|---|
Baryawno et al. [67] | Lin−Ter119−CD71− non-hematopoietic cells | Lepr-MSCs are significant source of Angpt1, Cxcl12, and Kitl New inferred osteoblast differentiation trajectories cause two different osteolineage subsets with distinct hematopoietic support potential A novel fibroblast subset expressing Cxcl12 and Angpt1 Arterial BMECs predominantly express high level of Kitl, Cxcl12 and Vwf compared to sinusoidal and arteriolar BMECs The relative proportions and the expression of hematopoiesis-regulatory factors in these key subsets are impaired by AML | N/A |
Wolock et al. [68] | CD45−Ter119− non-hematopoietic cells CD31− non-endothelial cells | HSC-supportive CAR cells can be mapped to a single MSC subset within the stroma Newly reconstructed differentiation paths from BM stroma to fat, bone, and cartilage | kleintools.hms.harvard.edu/paper_websites/bone_marrow_stroma |
Baccin et al. [69] | Lin−CD45−CD71−stroma cells Lin−cKit+ hematopoietic cells | Distinct niche residence cells are spatially allocated in the endosteal, sinusoidal, and arteriolar niches Cellular and spatial sources of cytokines to support HSCs Spatial relationships and intercellular signaling interactions of BM resident cell types Novel CAR cell subsets (i.e., Adipo-CAR cells in sinusoidal and Osteo-CAR cells in arteriolar endothelia) Adipo-CAR cells are main source of Cxcl12 and Kitl; Osteo-CAR cells are main source of Csf1 and Il7 Novel Ng2+ MSCs being placed at the apex of a differentiation hierarchy for all mesenchymal cell types | |
Tikhonova et al. [66] | Col2.3+ osteoblasts LepR+ perivascular cells VEcad+ vascular cells | The majority of niche cells are not actively cycling within quiescent BM microenvironment Adipocytic-primed LepR+ cells are preferential source of pro-hematopoietic factors 5-fluorouracil treatment induces cell proliferation across the niche subsets, and impacts adipo-lineage and osteo-lineage differentiation Vascular endothelium is the main sources of Notch ligands Dll4, which prevents the normal myeloid potential of hematopoietic progenitors The expression of vascular endothelial-specific Dll1 and Dll4 is downregulated under acute stress conditions | |
Zhong et al. [70] | Endosteal Td+ bone marrow cells | Age-dependent changes on the composite of mesenchymal populations and their bi-lineage differentiation routes in young, adult and aging mice A cluster of newly identified large population of adipogenic lineage precursors that regulate marrow vasculature and bone formation 3D networks constructed by Adipoq-labeled stromal cells and pericytes in bone marrow | N/A |
Tikhonova et al. [71] | – | An integrated overview of the bone marrow niche derived from five discussed scRNA-seq datasets combined | https://singlecell.broadinstitute.org/single_cell/study/SCP1248 |