Fig. 5

NK cell responses to HLA-I-modified allogeneic iPSC-ECs. A Schematic diagram of the experimental protocol for NK cell responses induced by co-culture with the HLA-I-modified allogeneic iPSC-ECs and measured by the degranulation marker CD107a and LDH release. B FCM analysis of CD107a on CD56+ NK cells: CD107a+ cells served as a readout for NK cell degranulation against the stimulation of different allogeneic iPSC-ECs. WT, KO and A11 groups described as in Fig. 4C. NK cells cultured alone were used as negative control (Neg.Ctrl); NK cells treated with PMA/ionomycin served as positive control (Pos.Ctrl). C Histograms of NK cell degranulation (n = 3 independent experiments). Paired one-way ANOVA followed by Tukey’ s multiple comparison test. Data are mean ± SEM; **P < 0.01. D LDH release assay of NK cytotoxicity against WT, KO, and A11 iPSC-ECs: histograms representing the percentage of NK cytotoxicity at the indicated effector/target (E/T) ratios (n = 3 replicates). Unpaired one-way ANOVA followed by Tukey’s multiple comparison test. Data are mean ± SD; *p < 0.05; ***p < 0.001