Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Matrix vesicles from dental follicle cells improve alveolar bone regeneration via activation of the PLC/PKC/MAPK pathway

Fig. 1

Characterization of human dental follicle cells (DFCs)-derived media matrix vesicles (MMVs) and collagenase-released matrix vesicles (CRMVs). A Transmission electron microscopy images of the ultrastructure. Scale bar:100 nm. Energy-dispersive X-ray analysis: B General views and distribution of calcium (Ca; blue) and phosphate (P; red) in MVs. Scale bar: 200 nm; C maps of the chemical composition of vesicles in detail. D Zeta-potential evaluation by the phase analysis light scatter (PALS). E Nanoparticle tracking analysis of particle size distribution. The red curve denotes CRMVs and blue for MMVs. F Western blot analysis of TSG101, HSP70, CD63, ALP and β-actin in MMVs, CRMVs and DFCs. G Confocal fluorescence microscopy detected the uptake of DiO-labeled MMVs or CRMVs (green) by DFCs after incubation for 6 h. Nuclei were stained with DAPI (blue). The cytoskeleton was stained with phalloidin (red). Scale bar: 20 μm

Back to article page