Fig. 3

Ricolinostat promoted MkP differentiation from HSPCs. A Flow cytometry analysis of changes in MkP (CD34⁺CD38⁺CD45RA⁻CD123⁻CD41a⁺) cell ratios. B Percentage of CD34⁺CD38⁺CD45RA⁻CD123⁻CD41a⁺ cells on day 7 of Ricolinostat treatment. C Cell number of CD34⁺CD38⁺CD45RA⁻CD123⁻CD41a⁺ cells from initial 10⁵ HSPCs on day 7 of Ricolinostat treatment. D CFU-MK were identified by staining with CD41 and subclassified as follows: small (3–20 cells); medium (20–50 cells); large (≥ 50 cells). Scale bars, 100 μm. E Absolute number of CFU-MK in different treatment conditions. F Expression analysis of some MkP-related genes in Ricolinostat-treated and -untreated cells on day 7 by qRT -PCR. Results are expressed as the mean ± SEM from three independent experiments. Unpaired Student’s t-test, *p < 0.05; **p < 0.01