Fig. 6

Ricolinostat might promote MkP differentiation by inhibiting IL-8 and its receptors. A Volcano map of differentially expressed genes. The abscissa is the fold change in gene expression in different experimental groups/different samples, and the ordinate is the significant degree of expression change. Blue represents significantly downregulated genes, and yellow represents significantly upregulated genes. Gray represents genes that were not significantly different. B Heat map of RNA-seq data for the indicated MK-related genes. C Gene Ontology analysis of downregulated genes in Ricolinostat-treated group compared to the untreated group. D KEGG analysis of downregulated genes in Ricolinostat-treated group compared to the untreated group. E qRT-PCR analysis of CXCR1, CXCR2, and IL-8 expression. F Expression of IL-8 in the culture supernatant on day 7 of Ricolinostat treatment measured by ELISA. G Flow cytometry analysis of CXCR1 expression on day 7 of Ricolinostat treatment. H Flow cytometry analysis of CXCR2 expression on day 7 of Ricolinostat treatment. Results are expressed as the mean ± SEM from three independent experiments. Unpaired Student’s t-test, **p < 0.01