Fig. 6
MSCCCR2 transplantation efficiently ameliorates inflammatory infiltration and hepatic apoptosis and promotes liver regeneration in the liver of ALF mice. A Hepatic macrophages level was detected using anti-F4/80 immunohistochemical staining of liver tissue sections from each group harvested at 36 h after TAA injection. Scale bar: 100 μm. Statistical analysis of the F4/80-positive cells in five random high-power fields of each section was performed. n = 5 per group from three independent experiments. B Hepatic neutrophils level was assessed by anti-Ly6G immunohistochemical staining of liver tissue sections from each group harvested at 36 h after TAA injection. Scale bar: 100 μm. Statistical analysis of the Ly6G-positive cells in five random high-power fields of each section was performed. n = 5 per group from three independent experiments. C The mRNA levels of proinflammatory cytokines TNF-α, IL-6, and IL-1β in livers from each group at 36 h after TAA injection were detected by qRT-PCR. GAPDH served as the internal control. n = 5 per group from three independent experiments. D Apoptosis level was detected using TUNEL (red) staining of liver tissue cryosections from each group harvested at 36 h after TAA injection. The nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. Statistical analysis of the percentage of TUNEL-positive cells in five random high-power fields of each section was performed. n = 5 per group from three independent experiments. E Hepatocyte proliferation was assessed by anti-Ki-67 immunohistochemical staining of liver tissue sections from each group harvested at 60 h after TAA injection. Scale bar: 100 μm. Statistical analysis of the Ki-67-positive cells in five random high-power fields of each section was performed. n = 5 per group from three independent experiments. All data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and n.s. means nonsignificant