Fig. 1

Effect of combined HX and ectopic differentiation on the myogenic capacities of SC in their microenvironment. a Immunofluorescence show SC positive for Pax7 at time 0 (T0 h), b MyoD at 48 h (T48) and c Myogenin at 72 h (T72) in red. SC/fiber were cultivated in basal medium (BM), myogenic differentiation (MD), adipogenic differentiation (AD) and osteogenic differentiation media up to 72 h under both normoxic (NX) and hypoxic (HX) culture conditions. d Quantification of the total number of MyoD positive cells per fiber (n = 20 fibers) at 48 h. e Quantification of cell clusters positive for MyoD per fiber at 48 h. f Quantification of the total number of cells positive for Myogenin per fiber (n = 20 fibers) at 72 h. g Quantification of cell clusters positive for Myogenin per fiber at 72 h. All data presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0. 001. DAPI was used as a nuclear counterstain (blue). Scale bar = 10 µM