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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice

Fig. 2

Effect of HX on quiescence and terminal differentiation of SC in their native substrate. a–j SC/fiber (n = 15–20) were cultivated in BM, MD, AD, OD media in 24-well culture plates in triplicate under both NX and HX conditions for 72 h. a Average percentage of Pax7 and Myogenin positive cells per fiber at 72 h. HX reduces the percentage of Myogenin positive cells and increased the percentage of Pax7 positive cells compared to NX condition. b–j A double immunofluorescence of SC/fiber show Myogenin (red) and Pax7 (green) positive cells (arrow). Note the cells positive for Myogenin (red, arrow head) and Pax7 (green, arrow head) under HX condition at 72 h (h–j). All data presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0. 001. DAPI was used as a nuclear counterstain (blue). Scale bar = 10 µM

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