Fig. 3

MenSC transplantation improved DDC-induced pathological changes in mouse livers. A H&E, Masson and Sirius Red staining of liver sections (n = 3 for each group). Scale bar: 250 μm. B Representative immunohistochemistry images for α-SMA and COL1A1 (n = 3 for each group). Scale bar: 250 μm. C Fibrosis area analysis measured from Masson and Sirius Red staining liver sections. D, E Semi-quantitative analysis of the expression of α-SMA and COL1A1 in liver. F Transmission electron microscopy of TJ in different groups. Scale bar: 0.5 μm for 25,000× and 200 nm for 50,000×. *p < 0.05, **p < 0.01, ***p < 0.001