Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Osteogenic differentiation of mesenchymal stem cells promotes c-Jun-dependent secretion of interleukin 8 and mediates the migration and differentiation of CD4+ T cells

Fig. 4

Osteogenic differentiation of MSCs decreases the percentage of Treg cells. Flow cytometry was used to detect Treg and Th17 cells in coculture systems. a, c MSCs significantly promoted the differentiation of Treg cells compared with anti-CD3/CD28 antibody stimulation alone, while the percentage of Treg cells in the osteogenic group was lower than that in the control group. b, d The differences in Th17 cells among the groups were not statistically significant. e The changes in the Treg/Th17 ratio were similar to the changes in the Treg percentage among the groups. f qRT-PCR analysis showed that the mRNA level of TGF-β1 increased when CD4+ T cells were cocultured with MSCs but decreased in the osteogenic group compared to the control group. h ELISA showed similar results. g, i Few significant differences in the expression of IL-17A were observed among the groups. Values are presented as the mean ± SD of 12 samples per group. ns indicated P > 0.05, and * indicates P < 0.05

Back to article page