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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Osteogenic differentiation of mesenchymal stem cells promotes c-Jun-dependent secretion of interleukin 8 and mediates the migration and differentiation of CD4+ T cells

Fig. 5

Secretion of IL-8 is elevated during MSC osteogenic differentiation. a Supernatants collected from MSC cultures were analyzed using the Human XL Cytokine Array Kit. b Several cytokines with differential expression including IL-8, ANG and ANG-1 were found. c, d Flow cytometry results showed that knockdown of IL-8 but not Ang or Ang-1 rescued the inhibitory effect on the Treg percentage, and a neutralizing antibody against IL-8 showed a similar effect. e, f An ARS assay showed that knocking down IL-8 had no effect on the osteogenic differentiation of MSCs. g Migration assays revealed that knocking down IL-8 exerted an inhibitory effect on the MSC-mediated migration of T cells. h A neutralizing assay showed a similar result. i Neutralizing antibody also weakened the migration effect of CD4+ T cells by MSCs. Values are presented as the mean ± SD of 12 samples per group. ns indicated P > 0.05, and * indicates P < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512