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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Osteogenic differentiation of mesenchymal stem cells promotes c-Jun-dependent secretion of interleukin 8 and mediates the migration and differentiation of CD4+ T cells

Fig. 6

c-Jun participates in the regulation of MSC osteogenic differentiation and IL-8 secretion. a qRT-PCR showed that the expression of c-Jun significantly increased during osteogenic differentiation. b Western blot analysis was performed to detect the protein level of c-Jun. c Binding effect was confirmed by luciferase reporter assays. d Western blot analysis showed that the protein level of IL-8 was regulated by c-Jun. e, g ARS assay results showed that knocking down c-Jun obviously weakened the osteogenic differentiation of MSCs. f, h An ALP assay showed a similar result. i Knocking down c-Jun weakened the migration of CD4+ T cells. j, k Flow cytometry results showed that knocking down c-Jun also rescued the inhibitory effect on the Treg percentage. Values are presented as the mean ± SD of 12 samples per group. ns indicated P > 0.05, and * indicates P < 0.05

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