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Table 2 Lineage-traced mouse lines specific to PEP lineages

From: Abnormal respiratory progenitors in fibrotic lung injury

Strain

Lineage

Markers

DATP specific lineages

  

Ndrg1CreER [10]

expressed in DATPs during alveolar regeneration

Ndrg1 lineage labeled cells emerged with a majority of Krt8+ cells in the alveolar region post-bleomycin injury (day 9). There were 30% of ATI cells derived from Ndrg1 lineage labeled cells post-bleomycin injury (day 28)

Krt8CreER [10]

Cldn4 + DATPs post-bleomycin injury

Krt8 expression was only detected in Cldn4+ DATPs post-bleomycin injury (day 9) in the alveolar region and was prominent in Pdpn+ ATI cells post-bleomycin injury (day 28)

Il1r1CreER [10]

expressed in airway ciliated cells and small subsets of mesenchyme cells in uninjured lungs

About 15% ATII cells were lineage labeled by Il1r1 in uninjured lung, Il1r1 lineage labeled ATII cell increased to 60% post-injury (day 14); 80% of the DATPs were lineage labeled by Il1r1

PATS specific lineages

Krt19CreER [12]

a transition population between ATII and ATI

Krt19 lineage labeled cells co-expressing ATI marker AGER were found 12 days post-bleomycin. No Krt19 lineage cells were found to give rise to Sftpc expressing ATII cells

Ctgf-GFP [12]

found in fibroblasts, but not in alveolar epithelial cells in normal lung

GFP expression in epithelial cells co-labeled by PATS markers Cldn4, Lgals3, and Sfn post-bleomycin injury and co-expressed low levels of ATII marker Sftpc

BCs

Krt17CreER [71]

transition population in the BC lineage

Krt17 lineage labeled cells showed limited expression pattern in BCs in developing trachea. About 90% of the Krt17 lineage labeled cells committed toward BC lineage at E14.5 and started expressing a mature BC gene signature Krt5 at E16.5

p63CreER [19]

Basal-like cells

It is reported that all arising Krt5+ cells post-influenza infection were lineage labeled by p63

Krt6-DTR [21]

Basal cells

This line enables the ablation of Krt6 expressing cells upon diphtheria toxin treatment. A study showed that 90% loss of Krt5+ cells and over 99% loss of Krt6+ cells were found in Krt6-DTR mouse lung compared to wild-type control 15 days post-influenza infection

Scgb3a2DreER [80]

Basal cells

This line enable lineage labeling of distal airway secretory cells, interlobar serous (IS) cells. This study showed IS cells became BCs following influenza infection