Fig. 5
From: Epimorphic regeneration of the mouse digit tip is finite
Attenuated regeneration of digits adjacent to repeatedly amputated digits. A–C Experimental outline of experiment 3 (A) (see “Methods” for description) and normalized bone volume (B) and length (C) of adjacent digits amputated for the first time (5th Amp MD; n = 18), compared to digits amputated for the 5th time (5th Amp, n = 36 digits), and digits of a separate mouse cohort of the same age amputated for the first time (1st Amp 6MO; n = 39 digits). D–F Mallory Trichrome-stained sections of age-matched control digits amputated for the first time (C), digits amputated for the 5th time (D), and adjacent middle digits amputated for the first time (E). Scale bar indicates 200 µm. Data are presented as mean ± SD and differences were determined using an unpaired t test. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001