Fig. 2
From: A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling
Age-related degenerated osteogenesis and angiogenesis were related to upregulated HIF1α and p53. A SA-β-gal staining in femurs and tibias. Bar = 250 μM. B Relative gene expression analysis of femurs and tibias. C, D Relative protein expression analysis of femurs and tibias. E–N Immunostaining analysis of HIF1α, p53, COL-1, VEGF and CD31 in femurs and tibias. Black/white bar = 100 μM; red bar = 25 Μm. MOD (mean optical densities) = Integral optical density/Area and MFD (mean fluorescence density) = Integral fluorescence density/Area. *p < 0.05 versus Young; #p < 0.05 versus Middle