Fig. 5
From: A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling
HIF1α showed opposite effects on the migration ability and osteogenesis of YBMSCs and ABMSCs, but no obvious effect on the cell proliferation. A, B, D, E Transwell assay of BMSCs interfered with HIF1α-siRNA or pCMV-HIF1α. Bar = 100 μM. C, F CCK-8 assay at 24 h and 48 h. G,L, ALP staining after 4-d osteogenic induction. H, M Relative gene expression of transfected BMSCs after 3-d osteogenic induction. I–K, N–P Relative protein expression of transfected BMSCs after 3-d osteogenic induction. *p < 0.05 versus YBMSC-NC or YBMSC-pCMV-blank; #p < 0.05 versus ABMSC-NC or ABMSC- pCMV-blank