Table 1 Primers used for RT-PCR
Gene | Type | Primers | Accession | Product length |
|---|---|---|---|---|
ALP | Forward | 5′-ACTGTGGACTACCTCTTG-3′ | XM_017346489 | 76 |
|  | Reverse | 5′-GGTCAGTGATGTTGTTCC-3′ |  |  |
BMP-2 | Forward | 5′-CGTGAGGATTAGCAGGTCTTTG-3′ | NM_001082650 | 127 |
|  | Reverse | 5′-TTTCGCTTGACGCTTTTCTC-3′ |  |  |
Osteocalcin | Forward | 5′- ACTCTTGTCGCCCTGCTG-3′ | XM_002715383 | 116 |
|  | Reverse | 5′-CTGCCCTCCCTCTTGGAC-3′ |  |  |
Runx2 | Forward | 5′-TCAGGCATGTCCCTCGGTAT-3′ | XM_017345160 | 54 |
|  | Reverse | 5′-TGGCAGGTAGGTATGGTAGTGG-3′ |  |  |
GAPDH | Forward | 5′-GCCTGGAGAAAGCTGCTAAGT-3′ | NM_001082253 | 133 |
|  | Reverse | 5′-GAGTGGGTGGCACTGTTGAA-3′ |  |  |