Fig. 1
From: Attenuates of NAD+ impair BMSC osteogenesis and fracture repair through OXPHOS
Distinct metabolic profiles in osteogenic and adipogenic committed BMSCs. A Seahorse mito-stress test for oxygen consumption rate (OCR) in BMSCs on Day 7. B Seahorse mito-stress test for extracellular acidification rate (ECAR) in BMSCs on Day 7. C Quantification of OCR parameters in BMSCs. D Energy map of BMSCs. Mean basal OCR in dependence of mean basal ECAR is shown. E Seahorse glycolytic test and quantification for ECAR in ST2 cells on Day 7. F Glucose consumption rate in BMSCs on Day 7. G Lactate production rate in BMSCs on Day 7. Ctrl: control; Osteo: osteogenesis; Adipo: adipogenesis; Oligo: oligomycin; FCCP: Trifluoromethoxy carbonylcyanide phenylhydrazone; Rot: rotenone; Ant: antimycin A. N = 3. Statistical significance was calculated with one-way ANOVA. *p < 0.05; **p < 0.01. Data are represented as mean ± SD