Fig. 2

Mitochondrial function is enhanced in osteogenic and adipogenic committed cells. A Representative image of JC-1 staining in ST2 cells at day 7. Right: quantification of red/green fluorescence intensity ratio in ST2 cells. Scale bar = 50 μm. N = 4. B Citrate synthase activity in ST2 cells at day 7. N = 3. C Representative image of western blot analysis with the ST2 cells. Quantification of the western blot from three independent experiments was shown upon the bands. N = 3. D Mito-tracker staining for ST2 cells at day 7. Top panel scale bar = 20 μm, bottom panel scale bar = 5 μm. N = 4. 3D: day 3; 7D: day 7. 14D: day 14. Statistical significance was calculated with two-way ANOVA for (A) and one-way ANOVA for (B). *p < 0.05; **p < 0.01. Data are represented as mean ± SD