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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Attenuates of NAD+ impair BMSC osteogenesis and fracture repair through OXPHOS

Fig. 4

FK866 inhibits bone fracture repair in vivo. A Representative H&E stain and IHC stain of Aggrecan and Osterix at 3 weeks post-fracture. Scale bar in low magnification: 200 μm, scale bar in high magnification: 50 μm. The blue dash line delineated the bone callus. B μCT images at 3 weeks post-fracture. Scale bar = 1 mm. C Bone parameters from fracture callus at 3 weeks post-fracture by μCT. N = 4. BMD: bone mineral density. BV: bone volume. TV: total volume. D Representative H&E stain and goldner trichrome stain at 3 weeks post-fracture. The blue dash line delineated the bone callus. The blue-coded boxed areas in the low magnification images (upper) were shown at high magnification in the corresponding boxes (lower). Top scale bar = 1 mm, bottom scale bar = 200 μm. N = 4. Statistical significance was calculated with Student’s t test. *p < 0.05; **p < 0.01. Data are represented as mean ± SD

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