Fig. 1

Temporospatial expression pattern of Mycn in tooth germ cells and verification of Mycn deletion in mutant mice. (A–E’) Immunofluorescence of Mycn (red) overlaid with DAPI (blue) at embryonic days 13.5 (E13.5) (A–A’), E14.5 (B–B’), E15.5 (C–C’), E16.5 (D–D’) and E18.5 (E–E’) of control mice. (F–G) Validation of the Cre activity (green) of Osr2^IresCre mice (F) at E15.5 and K14^Cre mice at E14.5 (G). (H–I’) Immunofluorescence of Mycn (red) overlaid with DAPI (blue) at E15.5 of control (H–H’) and Mycn^Osr2 mutant mice (I–I’). (J–K’) Immunofluorescence of Mycn (green) overlaid with DAPI (blue) at E14.5 of control (J–J’) and Mycn^K14 mutant mice (K–K’). Dotted lines indicate the epithelial compartment of the tooth germ. DE, dental epithelium; DP, dental papilla; OEE, outer enamel epithelium; IEE, inner enamel epithelium. Scale bars: 50 μm