Fig. 2
Mesenchymal ablation of Mycn results in thinner dentin in mandibular first molars and incisors. (A) Gross appearances show smaller mandibular molars and incisors of MycnOsr2 mutants compared with controls at PN1M. (B) Heatmap analysis of the X-ray radiograph shows distinctly lower dentin density of the MycnOsr2 mutant at PN6M. Red arrows point to the representative areas (the lingual side and apical area of incisor and the cementoenamel junction and dental cusp of molar) of decreased dentin greyscale. (C) Representative micro-CT scanning images and three-dimensional reconstructions (white: enamel; yellow: dentin) of control and mutant mice at PN6W. PCH: pulp chamber height. (D) Quantification of tooth, dentin and enamel volumes of mandibular first molars and incisors and pulp chamber height of the molars. *p < 0.05. ns not statistically significant