Fig. 3

Compromised differentiation of mDPCs of Mycn^Osr2 mutant mice. (A–B’) H&E staining shows fewer preodontoblasts (red arrows) in mutant mice at E17.5. (C–D’) H&E staining shows malformed odontoblasts (red arrows) and less predentin in mutant mice at P0. (E) The predentin area was significantly decreased in Mycn^Osr2 mutant mice at P0. (F–H) Alkaline phosphatase (ALP) staining and quantification at 9D of odontoblastic induction show decreased ALP activity in shMycn mDPCs. (I–K) Alizarin red staining (ARS) and quantification at 11D of odontoblastic induction show decreased calcium deposition in shMycn mDPCs. *p < 0.05