Fig. 4

Mycn directly binds to the promoter region of Klf4 and positively regulates its transcriptional activity. (A) Location of Mycn-occupancy peaks relative to the nearest annotated gene identified by CUT&Tag analysis. (B) Five most enriched sequence motifs at Mycn-occupancy sites as determined using HOMER. The top three matches of Motif #1 to known motifs are Sp1, Klf7 and Klf4. (C) Integrative genomics viewer (IGV) screenshots show tracks of normalized Mycn-occupancy tag counts. The red box identifies peaks bound by Mycn. The red arrow denotes the transcription start site (TSS) and transcription direction. (D) Dual luciferase assay shows that Mycn overexpression significantly upregulated the luciferase activity of the Klf4 promoter. *p < 0.05