Fig. 5

Mycn loss downregulated the expression of Klf4, Sp7 and Dmp1 in vivo. (A–D’) Representative immunofluorescence staining shows downregulation of Klf4 (A–B’) and Sp7 (C–D’) in mutants compared with the controls at E15.5. Dotted lines indicate the epithelial compartment of the tooth germ. (E–J’) Representative immunohistochemistry staining shows downregulation of Klf4 (E–F’), Sp7 (G–H’) and Dmp1 (I–J’) in mutants compared with the controls at P0. Scale bars: 50 μm. (K–O) Quantifications of the immunofluorescence and immunohistochemistry staining show significantly downregulated expression of Klf4, Sp7 and Dmp1 in mutants compared with the controls. *p < 0.05