Fig. 3.

Generation of hPGCLCs from ESCs in 2010s. A Schematic of hPGCLC specification in vitro. “Naive” hESCs were generated by treatment of hESCs with 4i for 3-5 days first, and then the hPSCs were preinduced when exposed to cytokines to further produce hPGCLCs [21] (top). hPSCs were induced to differentiate into iMeLCs in the presence of activin A and GSKi for 2 days. Then, iMeLCs were induced into hPGCLCs by cytokines, including BMP4, for 8 days [44] (bottom). 4i, ERKi, GSKi, JNKi, p38i,iMeLCs, incipient mesoderm-like cells. B Schematic of gamete specification from hPGCLCs in vitro. hPGCLCs cocultured with somatic cells from E12.5 embryonic gonads could further differentiate into oogonia precursor states [45].