Fig. 1
From: Modeling oxidative injury response in human kidney organoids
Hemin injury leads to induction of inflammation and nitric oxide mediated nitrosylation. a Representative quantitative PCR (qPCR) of kidney organoids treated with hemin at increasing concentrations. Expression relative to the control (untreated) organoids. b Time course qPCR of control and hemin treated organoids from day 15 to day 20. Results show mean and standard deviation of 3 technical replicates. c Immunofluorescence of kidney organoid paraffin sections of control and hemin treated organoids, showing localization of kidney injury marker 1 (KIM1, red) to proximal tubules labelled with Lotus tetragonolobus lectin (LTL, green), apoptotic marker activated caspase 3 (Casp3, red), DNA damage marker (γH2AX, green), heme catabolizing enzyme heme oxygenase 1 (HMOX1, orange), and nephrin labelling podocytes (NPHS1, red). d Immunofluorescence of kidney organoid paraffin sections at day 16, 18 and 20 showing 3-nitrotyrosine staining (red) indicative of nitrositive stress. Cell line MANZ2-2. Scale bar = 200 µm