Fig. 3
From: Modeling oxidative injury response in human kidney organoids
Assessment of proximal tubule transport function in kidney organoids. a Representative image of OAT (6CF) and OCT (EB) in the 12.5, 25 and 50 µM hemin treated kidney organoids. Note, transport declines with increasing concentrations of hemin. Each row shows the same corresponding organoid with 6CF, EB and overlay with transmitted light image. White arrow in 6CF control points to luminal accumulation in tubule structure. Maximum projections shown. Scale bar 100 µm. b Quantification of control vs 25 µM hemin treated kidney organoids showing changes between OAT (6CF) and OCT (EB) transport. Each dot represents one organoid from three independent assays and mean for each group is shown. One-way ANOVA with Dunnett’s T3 multiple comparisons test. ****P ≤ 0.0001. c Heat map showing expression in day 16 (D16) hemin (_H) and control (_C) samples of the OAT (SLC22A6, SLC22A8), OCT transporters (SLC22A2) and multi-drug resistant protein transporters (SLC47A1, SLC47A2, ABCC2). Cell line MANZ2-2