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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Modeling oxidative injury response in human kidney organoids

Fig. 4

CytoC-GFP biosensor localizes to the mitochondria. a Schematic representation of the safe harbor AAVS1 donor vector with CytoC-GFP construct. b Live immunofluorescence showing CytoC-GFP expression is colocalized to MitoTracker Red CMXRos labelled mitochondria in the iPSCs and kidney organoids. Mitochondrial expression in the tubule of the kidney organoids, and diffuse cytoplasmic staining in the menadione treated tubule. Scale bar = 10 µm. c Representative live images of 3D projections showing mitochondrial localization in the control and 25 µM hemin treated tubules of kidney organoids. Note the diffuse CytoC-GFP localization in the hemin treated tubule (white arrow). Data from isolated clone #8 CytoC-GFP iPSC line. Scale bars = 20 µm

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