Fig. 5
From: Modeling oxidative injury response in human kidney organoids
Treatment with UPHD25 reduces collagen induction in kidney organoids. a Representative images of paraffin sections of kidney organoids at day 26, stained with collagen hybridizing peptide (CHP; yellow) and collagen 1a1 antibody (COL1A1; green), DAPI staining nuclei (blue). UPHD25 concentrations 1, 3, and 9 µM. b Quantification of CHP staining. Each point represents CHP threshold area/DAPI per organoid, per section. c Quantification of COL1A1 staining. Each point represents COL1A1 threshold area/DAPI per organoid, per section. One-way ANOVA with multiple comparisons to hemin control. ns, non-significant; *P ≤ 0.05; ****P < 0.0001. Cell line MANZ2-2. Scale bar = 100 µm