Fig. 1

The rescue effects of MSC administration on APAP-induced liver failure are similar to those of NAC. a Morphology of in vitro-cultured MSCs. b Immunostaining and flow cytometry analysis revealed that MSCs did not express the haematopoietic stem cell marks CD34, CD45 and HLA-DR. c Immunostaining and flow cytometry analysis revealed that MSCs expressed CD105, CD44, CD90 and CD73. d Schematic representation of the experimental design to determine the effects of MSCs on APAP-induced liver failure. e Seven-day survival curve after APAP (500 mg/kg B.W.)-induced liver failure mice treated with PBS or MSCs (5 × 10⁵, 1 × 10⁶, 1.5 × 10⁶, 2 × 10⁶ cells) or NAC (300 mg/kg B.W.). f Serum ALT and AST levels at 24 h after APAP challenge. Data are expressed as the mean ± SD, and significant differences between groups are shown in red