Fig. 5

HGF is an essential cytokine of MSCs to protect L-O2 cells from APAP injury. a Intracellular staining and flow cytometry analysis of HGF, IL-6 and EGF in MSCs. b Western blot analysis showed the sustained expression of HGF, IL-6 and EGF in APAP-treated MSCs. c Giemsa staining of the surviving L-O2 cells co-cultured with MSCs and neutralizing antibodies against HGF, IL-6 and EGF in the presence of APAP (20 mM). d Annexin V and 7-AAD staining and flow cytometry analysis showed that HGF neutralization antibodies blocked the anti-necrotic effects of MSCs on APAP-treated L-O2 cells compared to IL-6 or EGF neutralization antibodies