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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The effect of mitochondrial fusion on chondrogenic differentiation of cartilage progenitor/stem cells via Notch2 signal pathway

Fig. 3

Mitochondrial fusion promoter M1 increased rCPSC differentiation toward chondrogenesis. Rat CPSCs were incubated with two different concentrations of MFP1 (5 and 10 µM). A The effect of MFP1 on mitochondrial fusion at mRNA level. B, C Western blot and quantitative analysis of MFP1 on mitochondrial fusion. D–F The role of MFP1 on chondrogenic and mitochondrial fusion markers at mRNA and protein levels during chondrogenic differentiation of CPSCs. G, H Safranin O and Alcian blue staining of CPSC pellet with or without MFP1. I Col2 levels detected by immunofluorescence staining. DAPI (blue), Col2 (green). Bar = 200 µM. The data are expressed as mean ± standard deviation. N = 3. *P less than 0.05 versus control group. Col2 = collagen II; MFN = mitofusin; MFP1 = mitochondrial fusion promoter M1

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