Fig. 4

The co-localization of Mfn2 and Prg4 in normal cartilage. A The co-localization of Mfn2 and Prg4 in normal articular cartilage. Mfn2 (green), Prg4 (red), DAPI (blue). B Immunofluorescence staining of Mfn2 and Prg4 at different OA induction times (1, 6 and 8 weeks). Mfn2 (green), Prg4 (red), DAPI (blue). C rCPSCs after lentiviral transfection observed under fluorescence microscope. GFP (green), DAPI (blue). Bar = 200 µM. D MFN2 mRNA levels significantly up- or down-regulated compared to the negative control groups. E, F Mfn2 protein levels significantly up- or down-regulated compared to the negative control groups, and quantitative analysis. The data are expressed as mean ± standard deviation, N = 3. *P less than 0.05 versus OE-NC. KD knockdown of Mfn2, KD-NC negative control of Mfn2 knockdown. OE over-expression of Mfn2, OE-NC negative control group of Mfn2 over-expression