Fig. 6

Mfn2- OE suppressed NOTCH2 signaling pathway. A Protein level expression of Notch2, smad3, smad4, and β-catenin on day 0, 3, 7 and 14 of chondrogenesis. B Relative quantitative analysis of the proteins level expression. C, D Comparison of the effects of relevant expression of Notch2, smad3, smad4, and β-catenin signaling pathways on undifferentiated CPSCs using western blot between OE-NC, OE, KD-NC, and KD group, and relative quantitative analysis. E, F Comparison of the effects of relevant expression of Notch2, smad3, smad4, and β-catenin signaling pathways on differentiated CPSCs using western blot between OE-NC, OE, KD-NC, and KD group, and relative quantitative analysis. The data are expressed as mean ± standard deviation, N = 3. *P less than 0.05 versus OE-NC, KD or KD-NC. OE over-expression of Mfn2, OE-NC negative control group of Mfn2 over-expression, KD knockdown of Mfn2, KD-NC negative control of Mfn2 knockdown