Table 1 Primer sequences used in this study
Gene | Accession number | Amplicon length (bp) | Forward | Reverse | Tm (°C) |
|---|---|---|---|---|---|
Rat Col2a1 | NM_012929.1 | 151 | GGCCAGGATGCCCGAAAATTA | CCCTCTCTCCCTTGTCACCAC | 61.5 |
Rat Acan | XM_039101035.1 | 196 | CTGGGTGGATGCAGAGAGAC | TTGGTTTGGACGCCACTTCT | 60.1 |
Rat Sox-9 | NM_080403.2 | 170 | AAGTCGGTGAAGAATGGGCA | GTCGGTGGACCCTGAGATTG | 60.11 |
Rat Mfn2 | NM_130894.4 | 118 | TCAAGCGCCAGTTTGTGGAG | CACAGATGAGCAAATGTCCCAGA | 60.17 |
Rat Mfn1 | NM_138976.1 | 136 | ATCTGGTGGAGATACAGGGCT | TCCCACAGCATTGCGTTGAT | 60.61 |
Rat Opa1 | NM_133585.3 | 108 | GGCACTTCAAGGTCGTCTCA | CACTGCTCTTGGGTCCGATT | 60 |
Rat Drp1 | NM_053655.3 | 94 | AGGTTGCCCGTGACAAATGA | CACAGGCATCAGCAAAGTCG | 60.18 |
Rat Fis1 | NM_001105919.1 | 108 | ACGCCTGCCGTTACTTCTTC | GCAACCCTGCAATCCTTCAC | 60.67 |
Rat 18S | NR_046237.2 | 172 | CCTGAGAAACGGCTACCACA | ACCAGACTTGCCCTCCAATG | 60.96 |